DNA transfection Mammalian cells Primary cells

Get tips on using YeaStar™ Genomic DNA Kit to perform DNA isolation / purification Yeast - Saccharomyces cerevisiae

Get tips on using QIAamp 96 DNA Blood Kit to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using QIAamp DNA Blood Mini Kit to perform DNA isolation / purification Yeast - Saccharomyces cerevisiae

Get tips on using QIAamp DNA Blood Midi Kit to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using QIAamp DNA Stool Mini Kit to perform DNA isolation / purification Yeast - Saccharomyces boulardii

Get tips on using MagAttract HMW DNA Kit (48) to perform DNA isolation / purification Bacteria - Gram negative Klebsiella

Get tips on using MagAttract PowerMicrobiome DNA/RNA EP to perform DNA isolation / purification Bacteria - Gram negative Klebsiella

Get tips on using QIAamp DNA Stool Mini Kit to perform DNA isolation / purification Bacteria - Gram positive Lactobacillus

Get tips on using Aquadien™ DNA Extraction Kit to perform DNA isolation / purification Bacteria - Gram negative Legionella

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.