sirna-rnai-mirna-transfection-human-hela-lipofectamine

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3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres Experiment

Cell culture media 3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres

Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells Experiment

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells MDA-MB-231 human breast cancer cells

Multiple gene silencing using ShRNA Discussion

Hello, can someone here help me? I am trying to silence e-selectin and ICAM-1 in endothelial cells. I would like to know if this is possible using shRNA

Discussions Multiple gene silencing using ShRNA

CytoSelect™ 24-Well Wound Healing Assay Product

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human HMEC