siRNA / miRNA gene silencing Human SKOV-3

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized Caov-3

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized OVCAR-3

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized OVCAR-3

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized bEnd.3

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized PC-3

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized Calu-3

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized BxPC-3

Get tips on using Mouse ANGPTL3 ELISA to perform ELISA Mouse - Angiopoietin-Like 3 (AngptL3)