Get tips on using Annexin V-FITC to perform Apoptosis assay cell type - PC-3
Get tips on using bisBenzimide H 33342 trihydrochloride to perform Apoptosis assay cell type - OVCAR-3
ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using pVRB2B3 to perform Protein Expression Eukaryotic cells - HEK293 hβ-defensin 2/3
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized SK-BR-3
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized OVCAR-3
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized OVCAR-3
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