Protein Expression Eukaryotic cells BHK cells

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Human neural progenitor cells (NPC)

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Cell Counting Kit-8 to perform Live / Dead assay mammalian cells - GH3

Get tips on using EZ4U - Cell Proliferation Assay to perform Live / Dead assay mammalian cells - GH3

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines PANC-1

Get tips on using NeuroMag to perform DNA transfection Mammalian cells - Immortalized cell lines SH-SY5Y

Get tips on using Autophagy Detection Kit to perform Autophagy assay cell type - Mesenchymal stromal cells

Get tips on using Iscove’s Modified Dulbecco’s Medium to perform Stem cell culture media hMegakaryoblastic cells

Get tips on using Minimum Essential Medium Eagle to perform Mammalian cell culture media HSG cells

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - Goblet cells