dna-isolation-purification-cells-primary-cells-mouse-embryonic-fibroblast-mef

Get tips on using Gentra Puregene Yeast/Bact. Kit to perform DNA isolation / purification Yeast - Candida parapsilosis

Get tips on using Gentra Puregene Yeast/Bact. Kit to perform DNA isolation / purification Yeast - Candida albicans

Get tips on using High Pure PCR Template Preparation Kit to perform DNA isolation / purification Tissue - placenta

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Blood / serum / plasma / buffy coat

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - MCF-7 human breast cancer cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - Cardiac fibroblasts

Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Mouse - Cardiac fibroblasts