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When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary mouse cardiomyocytes

Get tips on using SIRT1 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - PC3 (human prostate cancer cell line) SIRT1

Products Santa Cruz Biotechnology SIRT1 siRNA and shRNA Plasmids (h)

Get tips on using EpiTect 96 Bisulfite Kit (2) to perform Bisulfite DNA Modification Fluids

Products Qiagen EpiTect 96 Bisulfite Kit (2)

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - MCF10A

Products Thermo Fisher Scientific Qubit dsDNA HS Assay Kit

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - BJ

Products Thermo Fisher Scientific Qubit dsDNA HS Assay Kit

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - BMDM

Products Thermo Fisher Scientific Qubit dsDNA HS Assay Kit

Get tips on using 7-AAD (7-Aminoactinomycin D) to perform DNA quantification Human - BMDM

Products Thermo Fisher Scientific 7-AAD (7-Aminoactinomycin D)

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - HeLa

Products Thermo Fisher Scientific Qubit dsDNA HS Assay Kit

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Brain tissue

Products Thermo Fisher Scientific Qubit dsDNA HS Assay Kit

Get tips on using Silencer select_ObR siRNA to perform siRNA / miRNA gene silencing Human - MCF-7 ObR(leptin receptor/LEPR)

Products Thermo Fisher Scientific Silencer select_ObR siRNA

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