siRNA / miRNA gene silencing Rat Schwann cells

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Cell culture media 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

Cellular assays Cell line authentication Human iPSC cells derived from human dermal fibroblasts

Get tips on using QuikChange Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Rat - Point mutation H9C2 CELF1

Products Agilent Technologies QuikChange Site-Directed Mutagenesis Kit, 10 Rxn

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Rat - PC12

Products New England BioLabs NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

DNA DNA isolation / purification Cells Immortalized cell lines Human Neuroblastoma Cell Lines

The challenge in isolating RNA from S. aureus cells is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads is considered to be the better alternative.

RNA RNA isolation / purification Bacteria Gram positive Staphylococcus aureus

Proteins Protein expression and purification Mammalian cells HEK 293 HER2 leader peptide

Cell culture media 3D Cell Culture Media Human breast cancer MCF-7 cells-Mammospheres

Cell culture media 3D Cell Culture Media Mouse primary breast cancer ephitelial cells-Mammospheres

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Cellular assays ROS assay cell type A549 human adenocarcinomic human alveolar basal epithelial cells

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