siRNA / RNAi /miRNA transfection Mouse Glomerular Mesangial cells

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - C2C12

Get tips on using miRNeasy Micro Kit to perform RNA isolation / purification Cells - primary rat cortical neurons

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human cardiac fibroblasts

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized OS-RC-2

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - 3T3-L1