ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.
Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.
Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - SW480
Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - HaCaT
Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - HeLa
Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - HT-29
Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - HCT-116
Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - HL-60
Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - MCF 10A
Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - MCF-7
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment