Protein expression and purification Insect cells S2

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized C2C12

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized T98G

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Cells - immortalized HCA2

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized MDCK

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized CMT12

Get tips on using QIAamp Viral RNA Mini Kit to perform RNA isolation / purification Cells - immortalized Vero

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized Jurkat

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized A2780

Get tips on using Cell DNA Isolation Kit to perform DNA isolation / purification Cells - Immortalized cell lines C2C12

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.