siRNA / RNAi /miRNA transfection Rat Ar4-2j (Pancreatic tumor)

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human pulmonary artery smooth muscle cells

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human osteoblasts - rheumatoid arthritis

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human coronary artery smooth muscle cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary bovine pulmonary artery smooth muscle cells

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - primary porcine coronary artery endothelial cells

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - primary porcine coronary artery endothelial cells

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human carotid artery endothelial cells

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

Get tips on using DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #14793 to perform ChIP Anti-bodies FLAG