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Site Directed Mutagenesis (SDM) Rat

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Get tips on using SignalSilence® FoxO1 siRNA to perform siRNA / miRNA gene silencing Human - Calu-3 FOXO1

Products Cell Signaling Technology SignalSilence® FoxO1 siRNA

Get tips on using SignalSilence® IκBα siRNA to perform siRNA / miRNA gene silencing Human - Caco-2 IκBα

Products Cell Signaling Technology SignalSilence® IκBα siRNA

Get tips on using siRNA Junction plakoglobin (SASI_Hs01_00246520) to perform siRNA / miRNA gene silencing Human - BxPC-3 plakoglobin

Products Sigma-Aldrich siRNA Junction plakoglobin (SASI_Hs01_00246520)

Get tips on using Notch 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - A2780 Notch 1

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Get tips on using NGFR p75 siRNA (h) to perform siRNA / miRNA gene silencing Human - HUVEC NGFR p75

Products Santa Cruz Biotechnology NGFR p75 siRNA (h)

Get tips on using MEK-3 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - BMDMs MEK-3

Products Santa Cruz Biotechnology MEK-3 siRNA (m)

Get tips on using AMPKα1/2 siRNA (h) to perform siRNA / miRNA gene silencing Human - HepG2 AMPKα1/α2

Products Santa Cruz Biotechnology AMPKα1/2 siRNA (h)

Get tips on using CREB-2 siRNA (h) to perform siRNA / miRNA gene silencing Human - HUVEC ATF4 Lipid

Products Santa Cruz Biotechnology CREB-2 siRNA (h)

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type Hippocampal neural stem cells

Get tips on using TLR10 shRNA (h) Lentiviral Particles to perform shRNA gene silencing Human - THP-1 TLR10

Products Santa Cruz Biotechnology TLR10 shRNA (h) Lentiviral Particles

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