protein-isolation-tissue-mouse-lung-tissue

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Skin

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Pancreas

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Ovarian

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Muscle

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Liver

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Larynx

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Kidney

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Heart

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Adipose

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.