siRNA / miRNA gene silencing Human 501 Mel and SK Mel 28 FANCD2

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A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality hot-start DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction

DNA PCR Hot start PCR Bacterial DNA

Get tips on using MLH1 antibody [G168-15] to perform Immunohistochemistry Human - MLH1

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Get tips on using MessageAmp II aRNA Amplification Kit to perform Microarray RNA amplification & Labeling - Human brain tissue Cyanine 3

Products Thermo Fisher Scientific MessageAmp II aRNA Amplification Kit

Get tips on using MEBSTAIN Apoptosis TUNEL Kit Direct to perform TUNEL assay cell type - PANC-1 human pancriatic cancer

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Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Cellular assays Cell cycle assay mouse L929

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Cellular assays Cell cycle assay mouse C2C12

Get tips on using Gibco™Advanced DMEM/F-12 to perform Stem cell culture media Human intestinal stem cells/organoids

Products Thermo Fisher Scientific Gibco™Advanced DMEM/F-12

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Cellular assays Cell cycle assay mouse 3T3-L1

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Cellular assays Cell cycle assay mouse RAW 264.7

Does anyone know how “strong” the PCR product of methylation specific PCR is? I kept my PCR products at 4C for about 3 weeks and then at room temperature for another week. Will I be able to use them for sequencing?

Discussions How “strong” is the PCR product of methylation specific PCR?

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