DNA isolation / purification Bacteria

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - MRC5

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - HT1080

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - L929

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - U87MG

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - MG-63

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using s4215 to perform siRNA / miRNA gene silencing Human - SW1990 DNMT1/3b

Get tips on using s4215 to perform siRNA / miRNA gene silencing Human - PANC-1 DNMT1/3b

Get tips on using Hs_DNMT3B_2 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HCT-116 DNMT3B