rna-isolation-purification-cells-immortalized-cos-7

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

Get tips on using Autophagy Detection Kit to perform Autophagy assay cell type - Mesenchymal stromal cells

Get tips on using Iscove’s Modified Dulbecco’s Medium to perform Stem cell culture media hMegakaryoblastic cells

Get tips on using Minimum Essential Medium Eagle to perform Mammalian cell culture media HSG cells

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - Goblet cells

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - Goblet cells

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - K562 cells

Get tips on using Amino Allyl MessageAmp™ II aRNA Amplification Kit to perform Microarray RNA amplification & Labeling - Fish fundulus heteroclitus Cyanine-3 / Cyanine-5

Get tips on using pET30a(+)-karp to perform Protein Expression Prokaryotic cells - E. coli 56‐kDa O. tsutsugamushi strain Karp protein

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase