dna-quantification-human-pc-3

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using HighCell# ChIP kit to perform ChIP Rat - PCCL3

Get tips on using lentiCRISPR v2 to perform CRISPR Rat - Deletion PC12 MMP9

Get tips on using MCDB 131 Medium to perform Mammalian cell culture media PCAEC

Get tips on using Fabp5 siRNA to perform siRNA / miRNA gene silencing Rat - PC12 Fabp5

Get tips on using Vps13a siRNA to perform siRNA / miRNA gene silencing Rat - PC12 chorein

Get tips on using Rn_Sod2_4 FlexiTube siRNA to perform siRNA / miRNA gene silencing Rat - PC12 SOD2

Get tips on using Gibco™ DMEM, high glucose to perform Mammalian cell culture media PCAEC

Get tips on using Porcine Endothelial Cell Growth Medium to perform Mammalian cell culture media PCAEC

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Rat - Deletion PC12 Munc18