siRNA / RNAi /miRNA transfection Rat IEC

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using Stealth siRNA(r)_Mmp15 to perform siRNA / miRNA gene silencing Rat - C6 (rat glioma) mmp15

Get tips on using PICK1 siRNA to perform siRNA / miRNA gene silencing Rat - Astrocytes PICK1

Get tips on using Fabp5 siRNA to perform siRNA / miRNA gene silencing Rat - PC12 Fabp5

Get tips on using Vps13a siRNA to perform siRNA / miRNA gene silencing Rat - PC12 chorein

Get tips on using Gabpb1 siRNA to perform siRNA / miRNA gene silencing Rat - Schwann cells Nrf2

Get tips on using Ccr2 siRNA to perform siRNA / miRNA gene silencing Rat - Glial cells CCR2

Get tips on using Nrf2 siRNA (r) to perform siRNA / miRNA gene silencing Rat - NRCM Nrf2