RNA isolation / purification Cells Cancer cell lines

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized MIA PaCa-2

Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Bacteria - Gram negative Haemophilus influenzae

Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Bacteria - Gram negative Hemophilus influenzae

Get tips on using EZ-RNA Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Brain

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized 59M

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780ADR

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - primary human preadipocytes