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Cell cytotoxicity / Proliferation assay

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Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - mouse C166

Products Cell Biolabs CytoSelect™ 24-Well Wound Healing Assay

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - mouse 4T1

Products Cell Biolabs CytoSelect™ 24-Well Wound Healing Assay

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human HMEC

Products Cell Biolabs CytoSelect™ 24-Well Wound Healing Assay

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human HUVEC

Products Cell Biolabs CytoSelect™ 24-Well Wound Healing Assay

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - H9c2 rat cardiomyocytes

Products Abcam DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - K562 human leukemia cells

Products Abcam DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit

Get tips on using Mitochondrial ROS Activity Assay Kit (Deep Red Fluorescence) to perform ROS assay cell type - mouse cardiomyocytes

Products eEnzyme Mitochondrial ROS Activity Assay Kit (Deep Red Fluorescence)

Get tips on using Apo-ONE® Homogeneous Caspase-3/7 Assay to perform Apoptosis assay cell type - SH-SY5Y

Products Promega Apo-ONE® Homogeneous Caspase-3/7 Assay

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells HUVEC

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells K562

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