RNA sequencing Rat

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized MC-38

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized Caco-2

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized BxPC-3

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized HEK 293

Get tips on using TRIzol™ Plus RNA Purification Kit to perform RNA isolation / purification Cells - primary rabbit aortic endothelial cells

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Bacteria - Gram negative Bordetella pertussis

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - primary mouse cortical neurons

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized MIA PaCa-2

Get tips on using REALTOTAL RNA Spin Plus to perform RNA isolation / purification Cells - primary human aortic smooth muscle cells

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.