cell-cycle-assay-human-hela

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - mouse dorsal skin tissue

Get tips on using Cellular Senescence Flow Cytometry Assay to perform Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)

Get tips on using Beta-Galactosidase Assay to perform Reporter gene assay β-galactosidase substrates - INS-1 832/13

Get tips on using ApopTag® Peroxidase In Situ Apoptosis Detection Kit to perform TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - PANC-, BxPC-3 human pancreas

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - PC-3 human prostate adenocarcinoma

Get tips on using CellROX™ Deep Red Reagent, for oxidative stress detection to perform ROS assay cell type - PANC-, BxPC-3 human pancreas

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using QIA33 | FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme to perform Apoptosis assay cell type - Human endometrial stromal cells