siRNA / miRNA gene silencing Human hES cell line H1 (WA01)

Get tips on using Human Melanocyte Media to perform Mammalian cell culture media HEM

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - HeLa

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - SK-Hep-1

Get tips on using IncuCyte® Cell Migration Kit to perform Wound healing assay cell type - human MEWO

Get tips on using IncuCyte® Cell Migration Kit to perform Wound healing assay cell type - human A375

Get tips on using Cell Comb™ Scratch Assay to perform Wound healing assay cell type - human BEAS2B

Get tips on using Cell Comb™ Scratch Assay to perform Wound healing assay cell type - human A549

Get tips on using IncuCyte® Cell Migration Kit to perform Wound healing assay cell type - human HUVEC

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - Cancer cell lines Liver cancer cell lines Hepato cellular carcenoma (SMMC-7721, Huh7 & HepG2))

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.