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Acid phosphatase assay

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Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - human fibroblast tissue

Products Thermo Fisher Scientific LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - L29 mouse fibroblast

Products Thermo Fisher Scientific LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Get tips on using The Premo Autophagy Tandem Sensor RFP-GFP-LC3B Kit to perform Autophagy assay cell type - Human osteosarcoma cancer cells

Products Thermo Fisher Scientific The Premo Autophagy Tandem Sensor RFP-GFP-LC3B Kit

Get tips on using FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme to perform Apoptosis assay cell type - Human endometrial stromal cells

Products Millipore FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme

Get tips on using LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry to perform Live / Dead assay yeast - Saccharomyces cerevisiae

Products Thermo Fisher Scientific LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry

Get tips on using LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry to perform Live / Dead assay yeast - Candida albicans

Products Thermo Fisher Scientific LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

DNA Plasmid Isolation Pediococcus acidilactici

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells

Products Promega DeadEnd™ Colorimetric TUNEL System

Get tips on using Live-Dead Cell Staining Kit (BioVision) to perform Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells

Products Biovision Live-Dead Cell Staining Kit (BioVision)

Get tips on using LIVE/DEAD™ Cell Imaging Kit to perform Live / Dead assay mammalian cells - MDA-MB-231 human breast cancer cells

Products Thermo Fisher Scientific LIVE/DEAD™ Cell Imaging Kit

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