Site Directed Mutagenesis (SDM) Human Deletion MCF-7

Get tips on using siRNA FOXL2 to perform siRNA / miRNA gene silencing Human - COV-434 FOXL2

Get tips on using p53 siRNA to perform siRNA / miRNA gene silencing Human - COV-434 p53

Get tips on using siRNA EHF to perform siRNA / miRNA gene silencing Human - Calu-3 EHF

Get tips on using siRNA SRC to perform siRNA / miRNA gene silencing Human - CAL-27 SRC

Get tips on using siRNA ILK to perform siRNA / miRNA gene silencing Human - CAL-27 ILK

Get tips on using siRNA ITGAV to perform siRNA / miRNA gene silencing Human - CAL-27 ITGAV

Get tips on using siRNA RBM3 to perform siRNA / miRNA gene silencing Human - BxPC-3 RBM3

Get tips on using siRNA RIP3 to perform siRNA / miRNA gene silencing Human - BxPC-3 RIP3

Get tips on using siRNA FOXM1 to perform siRNA / miRNA gene silencing Human - BT-20 FOXM1

Stem cells have the unique ability to self-renew or differentiate themselves into various cell types in response to appropriate signals. These cells are especially important for tissue repair, regeneration, replacement, or in the case of hematopoietic stem cells (HSCs) to differentiate into various myeloid populations. Appropriate signals refer to the growth factor supplements or cytokines that mediate differentiation of various stem cells into the required differentiated form. For instance, HSCs can be differentiated into dendritic cells (with IL-4 and GM-CSF), macrophages (with m-CSF) and MDSCs (with IL-6 and GM-CSF). Human pluripotent stem cells (hPSCs) and induced pluripotent stem cells (iPSCs) can be first cultured in neural differentiation media (GSK3𝛃-i, TGF𝛃-i, AMPK-i, hLIF) to form neural rosettes, which can be differentiated into neural or glial progenitors (finally differentiated into oligodendrocytes). Neural progenitors can be finally differentiated into glutaminergic (dibytyryl cAMP, ascorbic acid) and dopaminergic (SHH, FGF-8, BDNF, GDNF, TGF-𝛃3) neurons. Thus, it is important to first identify the self-renewing cell line: its source and its final differentiation state, followed by the supplements and cytokines required for the differentiation, and final use. Timelines are another thing that is considered. For instance, it takes 7-10 days to form neural rosettes from iPSCs and 3 days to differentiate neural progenitors to neurons. Finally, the stability for stem cell culture media varies. It is advised to make fresh media every time when differentiating HSCs to myeloid populations, whereas neural differentiation media may remain stable for two weeks when stored in dark between 2-8C.