siRNA / RNAi /miRNA transfection Human Cells OV-2008

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized BEAS-2B

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized A2780/DDP

Get tips on using miRNeasy Micro Kit to perform RNA isolation / purification Cells - primary rat cortical neurons

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized OS-RC-2

Get tips on using ApopTag® Peroxidase In Situ Apoptosis Detection Kit to perform TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells

Get tips on using Click-iT™ TUNEL Alexa Fluor™ 488 Imaging Assay to perform TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,