siRNA / RNAi /miRNA transfection Human Cells HNSCC

Get tips on using Lipofectamine® RNAiMAX Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - Jurkat cells Lipofectamine

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - HeLa Lipofectamine

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - HESC Lipofectamine

Get tips on using Lipofectamine® RNAiMAX Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - OV-2008 Lipofectamine

Get tips on using Lipofectamine® RNAiMAX Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - THP-1 Lipofectamine

Get tips on using Lipofectamine® RNAiMAX Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - NK-92 Lipofectamine

Get tips on using Lipofectamine® RNAiMAX Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - KG-1 Lipofectamine

Get tips on using TransIT-TKO Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - Primary splenocytes Polymer / lipid

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.