DNA methylation profiling Gene specific profiling MRC-5

Get tips on using Live or Dead™ Cell Viability Assay Kit *Green/Red Dual Fluorescence to perform Live / Dead assay mammalian cells - rat endothelial progenitor cells

Get tips on using pAC2-dual-dCas9VP48-sgExpression to perform CRISPR Human - Activation IL1RN

Get tips on using pAC2-dual-dCas9VP48-sgExpression to perform CRISPR Human - Activation SOX2

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Live / Dead assay mammalian cells - MC3T3

Get tips on using LC3B antibody to perform Autophagy assay cell type - NRK-52E

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized 59M

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized Detroit 562

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized RIN-5F

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized K-562