Protein expression and purification Mammalian cells HeLa

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Heart

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Human umbilical cord tissue

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Rabbit eye retina/choroids

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human gHMVEC (glioma human microvascular endothelial cells)

Get tips on using CRP (Human) ELISA Kit (KA0238) to perform ELISA Human - C-Reactive Protein/CRP

Get tips on using TAGZyme Qcyclase/pGAPase Enzymes (150 U) to perform Protein tag His-tag removal

Get tips on using Trichloroacetic Acid Solution, MP Biomedicals™ to perform Protein isolation Bacteria - Borrelia burgdorferi

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Heart