siRNA / miRNA gene silencing Human Melanoma cells (501 Mel and SK Mel 28)

Get tips on using CM-H2DCFDA (General Oxidative Stress Indicator) to perform ROS assay cell type - PC12

Get tips on using CM-H2DCFDA (General Oxidative Stress Indicator) to perform ROS assay cell type - T47D

Get tips on using CM-H2DCFDA (General Oxidative Stress Indicator) to perform ROS assay cell type - CHO-K1

Get tips on using CM-H2DCFDA (General Oxidative Stress Indicator) to perform ROS assay cell type - 3T3-L1

Get tips on using LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation to perform Live / Dead assay mammalian cells - rat testicular tissue

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?