wound-healing-assay-cell-type-human-mcf-7

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

There are a plethora of detection methods of cell cytotoxicity and proliferation by flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - rat MSC

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - mouse C166

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - mouse 4T1

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - mouse NIH 3T3

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Cell migration / Invasion cell type - HaCat

Get tips on using Oris™ Universal Cell Migration Assembly Kit, 96 wells to perform Wound healing assay cell type - human MCF-7

Get tips on using Oris™ Cell Migration Assay to perform Wound healing assay cell type - human MCF-10A