siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells hsa-miR-542-3p

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Get tips on using pSilencer 2.1-U6 Hygro to perform shRNA gene silencing Human - Neuroblastoma cells (SH-SY5Y) Beclin 1

Products Thermo Fisher Scientific pSilencer 2.1-U6 Hygro

Get tips on using Lipofectamine® RNAiMAX Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - OV-2008 Lipofectamine

Products Thermo Fisher Scientific Lipofectamine® RNAiMAX Transfection Reagent

Get tips on using TransIT®-LT1 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - HNSCC Polymer / Lipid

Products Mirus TransIT®-LT1 Transfection Reagent

Get tips on using Lipofectamine® RNAiMAX Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - THP-1 Lipofectamine

Products Thermo Fisher Scientific Lipofectamine® RNAiMAX Transfection Reagent

Get tips on using Lipofectamine® RNAiMAX Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - NK-92 Lipofectamine

Products Thermo Fisher Scientific Lipofectamine® RNAiMAX Transfection Reagent

Get tips on using Lipofectamine® RNAiMAX Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - KG-1 Lipofectamine

Products Thermo Fisher Scientific Lipofectamine® RNAiMAX Transfection Reagent

Get tips on using 7ml p53 Bond RTU Primary to perform Immunohistochemistry Human - p53

Products Leica 7ml p53 Bond RTU Primary

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Primary cells Human primary keratinocytes

Products Promega Wizard® Genomic DNA Purification Kit

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-lactamase substrates HEK 293 & HEK 293T cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates U87 and U251 glioblastoma cells

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