Protein expression and purification Mammalian cells

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Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse lung tissue

Products Sigma-Aldrich CelLytic™ MT Cell Lysis Reagent

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - ME epithelial tissue

Products Sigma-Aldrich CelLytic™ MT Cell Lysis Reagent

Get tips on using CelLytic™ NuCLEAR™ Extraction Kit to perform Protein isolation Tissue - Mouse cardiac tissue

Products Sigma-Aldrich CelLytic™ NuCLEAR™ Extraction Kit

Get tips on using Tissue Extraction Reagent I to perform Protein isolation Tissue - Mouse liver tissue

Products Thermo Fisher Scientific Tissue Extraction Reagent I

Get tips on using TAGZyme DAPase Enzyme (50 U) to perform Protein tag His-tag removal

Products Qiagen TAGZyme DAPase Enzyme (50 U)

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Apoptosis assay cell type Array of apoptotic proteins

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Human umbilical cord tissue

Products Sigma-Aldrich CelLytic™ MT Cell Lysis Reagent

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Rabbit eye retina/choroids

Products Sigma-Aldrich CelLytic™ MT Cell Lysis Reagent

Get tips on using CRP (Human) ELISA Kit (KA0238) to perform ELISA Human - C-Reactive Protein/CRP

Products Abnova CRP (Human) ELISA Kit (KA0238)

Get tips on using TAGZyme Qcyclase/pGAPase Enzymes (150 U) to perform Protein tag His-tag removal

Products Qiagen TAGZyme Qcyclase/pGAPase Enzymes (150 U)

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