Protein expression and purification Mammalian cells HEK 293

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Breast

Get tips on using RNAqueous™ Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Uterus

Get tips on using Aurum™ Total RNA Mini Kit to perform RNA isolation / purification Tissue - Human Subcutaneous

Get tips on using MagNA Pure Compact RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Placenta

Get tips on using MEDOX-Easy Total RNA Extraction Reagent to perform RNA isolation / purification Tissue - Human Mouth

Get tips on using MagNA Pure Compact RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Bronchi

Get tips on using EZ-RNA Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Brain

Get tips on using MagAttract 96 DNA Plant Core Kit (24) to perform DNA isolation / purification Plants - Leaves

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.