siRNA / miRNA gene silencing Human HT-29

- Found 5266 results

siRNA EHF Product

Get tips on using siRNA EHF to perform siRNA / miRNA gene silencing Human - Calu-3 EHF

Products Thermo Fisher Scientific siRNA EHF
siRNA SRC Product

Get tips on using siRNA SRC to perform siRNA / miRNA gene silencing Human - CAL-27 SRC

Products Thermo Fisher Scientific siRNA SRC
siRNA ILK Product

Get tips on using siRNA ILK to perform siRNA / miRNA gene silencing Human - CAL-27 ILK

Products Thermo Fisher Scientific siRNA ILK

Get tips on using siRNA ITGAV to perform siRNA / miRNA gene silencing Human - CAL-27 ITGAV

Products Thermo Fisher Scientific siRNA ITGAV
siRNA RBM3 Product

Get tips on using siRNA RBM3 to perform siRNA / miRNA gene silencing Human - BxPC-3 RBM3

Products Thermo Fisher Scientific siRNA RBM3
siRNA RIP3 Product

Get tips on using siRNA RIP3 to perform siRNA / miRNA gene silencing Human - BxPC-3 RIP3

Products Santa Cruz Biotechnology siRNA RIP3

Get tips on using siRNA FOXM1 to perform siRNA / miRNA gene silencing Human - BT-20 FOXM1

Products Sigma-Aldrich siRNA FOXM1

Get tips on using ON-TARGETplus Human BSG / emmprin (682) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BCP-1 Emmprin / BSG

Products Dharmacon ON-TARGETplus Human BSG / emmprin (682) siRNA - SMARTpool

Get tips on using ON-TARGETplus Human PPRC1 siRNA to perform siRNA / miRNA gene silencing Human - MCF-7 PRC (PGC-1α–related coactivator)/PPRC1

Products Horizon Discovery Ltd. ON-TARGETplus Human PPRC1 siRNA

Short hairpin or small hairpin RNA (shRNA) is artificial RNA, which has a hairpin loop structure, and uses inherent microRNA (miRNA) machinery to silence target gene expression. This is called RNA interference (RNAi). These can be delivered via plasmids or viral/bacterial vectors. Challenges in shRNA-mediated gene silencing include 1. Off-target silencing, 2. Packaging shRNA encoding lentivirus, and 3. Stable transduction in cells. RNAi has been designed to have anywhere from 19-27 bs, but the most effective design has 19 bp. In case commercial shRNAs are not available, potential target sites can be chosen within exon, 5’- or 3’ UTR, depending on which splice variants of the gene are desired. One should use the latest algorithms and choose at least two different sequences, targeting different regions, in order to have confidence in overcoming off-target effects. A BLAST search after selecting potential design will eliminate potential off-target sequences. For the second challenge, sequencing the vector using primers for either strand (50-100 bp upstream) is suggested, along with using enzymatic digestion on agarose gel for the vector. Next, once the shRNA-containing vector is packaged in a virus, it is important to check the viral titer before transduction. Finally, using a marker in the lentiviral vector (fluorescent protein or antibiotic resistance), along with qPCR for target gene expression can help in determining the efficacy of transduction and shRNA on its target site.

RNA shRNA gene silencing Human SiHa AEG-1

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